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KMID : 0620920180500110142
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Experimental & Molecular Medicine
2018 Volume.50 No. 11 p.142 ~ p.142
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Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression
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Lee Mi-Nam
Hwang Hee-Su
Oh Sin-Hye
Roshanzadeh Amir
Kim Jung-Woo
Song Ju-Han
Kim Eung-Sam
Koh Jeong-Tae
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Abstract
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Supplementation of mesenchymal stem cells (MSCs) at sites of bone resorption is required for bone homeostasis because of the non-proliferation and short lifespan properties of the osteoblasts. Calcium ions (Ca2+) are released from the bone surfaces during osteoclast-mediated bone resorption. However, how elevated extracellular Ca2+ concentrations would alter MSCs behavior in the proximal sites of bone resorption is largely unknown. In this study, we investigated the effect of extracellular Ca2+ on MSCs phenotype depending on Ca2+ concentrations. We found that the elevated extracellular Ca2+ promoted cell proliferation and matrix mineralization of MSCs. In addition, MSCs induced the expression and secretion of osteopontin (OPN), which enhanced MSCs migration under the elevated extracellular Ca2+ conditions. We developed in vitro osteoclast-mediated bone resorption conditions using mouse calvaria bone slices and demonstrated Ca2+ is released from bone resorption surfaces. We also showed that the MSCs phenotype, including cell proliferation and migration, changed when the cells were treated with a bone resorption-conditioned medium. These findings suggest that the dynamic changes in Ca2+ concentrations in the microenvironments of bone remodeling surfaces modulate MSCs phenotype and thereby contribute to bone regeneration.
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KEYWORD
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Cell signalling, Mesenchymal stem cells
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